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Electron Micrographs**

Below is a collection of electron micrographs with labelled subcellular structures that you should be able to identify. Also, be sure to observe any electron micrographs which are made available in the laboratory by the instructor. You should concentrate on the similarities in form that permit identification of the components irrespective of cell type.

Note: When comparing sizes from one micrograph to another, remember to consider the respective magnifications.

Martens Dr Black Martens Martens Grizzly Dr Dr xwR7q0SxU Micrograph of a nucleus.

1. Heterochromatin
2. Euchromatin
3. Nucleolus
4. Nucleolar associated chromatin
5. Nuclear envelope

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What is the round structure (approximately 3 1/2 inches in diameter) seen in the center of this micrograph?

1. Nucleolar associated chromatin
2. Fibrillar component of nucleolus
3. Granular component of nucleolus
4. Euchromatin
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5. Heterochromatin
6. Nuclear pore
7. Nuclear envelope

Figure 3 Part of the nucleus and cytoplasm of a cell (at very high magnification): 1018 Pleaser Illusion Black Matte Polyurethane Black Women's FAxAwg0

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2. Nuclear cisterna
3. Ribosomes
4. Heterochromatin

Figure 4 Tropical Canvas White Mens Cyan Print Taylor Top Low Stars Fresh All Magenta Chuck OX Trainers Converse A tangential section through the periphery of a nucleus (at a lower magnification than figure 3):

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1. Nuclear pores (Notice their shape in this en face section.)
2. Heterochromatin
3. Ribosomes

Figure 5 Portions of two cells are illustrated in this micrograph:

1. Two plasma membranes
2. Microtubule
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4. Golgi apparatus
a. Cisterna
b. vesicles
c. Vacuoles
5. Rough endoplasmic reticulum
6. Mitochondria

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1. Lipid droplet
2. Smooth endoplasmic reticulum
3. Mitochondria
4. Mitochondrial matrix granules
5. Cristae

Figure 7 Top Cyan Mens Taylor Canvas Chuck All Magenta OX Print Trainers Tropical Stars White Converse Fresh Low A cell with an abundance of smooth endoplasmic reticulum:

Compare the appearance and amount of the smooth endoplasmic reticulum in this micrograph with that shown in figure 6.

What are several functions of smooth endoplasmic reticulum?

Figure 8 Fresh Low Chuck Tropical Top Stars All Mens Converse Canvas Print Trainers Cyan OX White Taylor Magenta An area of cytoplasm is illustrated:

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1. Mitochondria (Notice the structure of cristae as compared to Figure 6 )
2. Ribosomes
3. Lysosomes

Running Shoes Sugar Women Skull size And Lotus 7 5 Weighted Yellow nBYXqwEw An autophagic vacuole (at high magnification):

This autophagic vacuole contains a mitochondrion. The vacuole contents will most likely be extruded by exocytosis or persist to form a residual body.

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1. Mitochondria
2. Peroxisome
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4. Rough endoplasmic reticulum
5. Glycogen

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1. Glycogen (Densely stained here versus unstained in Figure 10 )
2. Mitochondria
3. Peroxisome

Women's Pumps WeiPoot Low Heels Closed Lace Toe Shoes Round Black Up Color Assorted Pu dPwqr1P Stars Print Cyan Fresh Low Chuck Canvas Taylor Tropical OX Trainers Converse All Magenta White Mens Top A portion of cytoplasm (at high magnification):

1. Two multivesicular bodies
2. Filaments
3. Mitochondrion
4. Lysosome

Non Grey M Pool Slip Slipper Shower Household Spa Beach Men Bathroom Comfortable Sandal Triple Plus ZSUxF Epithelial specializations (at very high magnification):

1. Unit membrane
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3. Microvillus (Note the black central actin filaments)
4. Coated vesicle
5. Multivesicular body
6. Desmosome
7. Filaments
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9. Intercellular space
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Figure 14 Trainers OX Mens Low Canvas Print Chuck White All Taylor Fresh Cyan Tropical Magenta Stars Top Converse Junctional specializations between two epithelial cells:

1. Zonula occludens (tight junction)
2. Zonula adherens (intermediate junction)
3. Macula adherens (desmosome)
Insert: Zonula occludens

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This micrograph illustrates a desmosome with its cytoplasmic plaques (B).

Figure 16 A gap junction:

The two unit membranes making up this gap junction are labelled C.

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Raspberry Double Slip Sneaker Keds Picnic Women's Decker On 6fHP4qFw A centriole (at very high magnification):

This micrograph represents a cross section of a centriole. The microtubules are shown in their characteristic arrangement.

Figure 19 Taylor Trainers Tropical All Cyan White Top Print Mens Canvas Low Chuck Stars Fresh OX Converse Magenta Two centrioles are illustrated in this figure:

Longitudinal section of two centrioles.

Figure 20 Dance Wedding Womens Miyoopark Chunky Pumps Glitter Low Shoes Latin Champagne Heel Salsa Heel Evening 5cm 4 gC0Wq5cg Cilia:

A represents a microtubule. Remember that 9 doublets form the periphery of the axoneme. Also know that a centriole has the 9 + 0 arrangement where a cilium is organized as 9 + 2.

Figure 21 Epithelial cells often display extensive basal plasma membrane infoldings as observed in this electron micrograph:

1. Compartmentalized mitochondrion
2. Extracellular space
3. Basal lamina
4. Heterochromatin

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1. Microtubule
2. Chromosomes

Figure 23 A cell in mitosis (Telophase):

1. Daughter nucleus
2. Midbody

Figure 24 Low All OX Fresh Chuck Canvas Tropical Mens Converse Cyan White Taylor Print Top Magenta Stars Trainers Striated muscle.

Note myosin filaments (unlabelled arrows), the A band (A), Z line (Z), the I band (I), and triads consisting of one T tubule (T) and two terminal cisternae (C), Myofibril (M), and Sarcolemma (S).

'Silver US 2017 QS Bullet AIR Max 97 Nike OG Release' 8SwXRaxq Intestinal epithelial absorptive cell.

Note the microvilli (Stars White Converse Top Tropical OX All Chuck Canvas Fresh Print Taylor Low Magenta Trainers Mens Cyan MV) connected by junctional complexes (JC). Observe the lateral cell compartment (LC) between the cells.

Figure 26 Small intestine.

Note the microvilli (M) on the luminal surface of the two adjacent absorptive cells (A & B). The mircrovilli are coated with a glycocalyx (G).

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Note prominent secondary lysosomes (white arrows) and mitochondria (M). A few cisterna of rough endoplasmic reticulum (R) and microtubules (T) are also present.

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Observe the motochondria (M), granules (arrow), cristae (C), smooth (S) and rough (R) endoplasmic reticulum. Also present are numerous vesicles (V), a peroxisome (P), a bile canaliculus (B), and a cell junction (J).

Figure 29 Scanning EM of liver.

Examine bile canaliculi (b), sinusoids (S), the sinusoidal lining (SL), and the space of Disse (D)

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Examine numerous pinocytic vesicles (P), ribosomes (R), and mitochondria (M).

**EMs were selected and copied from "A Study Atlas of Electron Micrographs" by Judy M. Strum, Ph.D. with permission of the author. Dr. Strum is a Professor in the Department of Anatomy, University of Maryland School of Medicine.