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Electron Micrographs**

Below is a collection of electron micrographs with labelled subcellular structures that you should be able to identify. Also, be sure to observe any electron micrographs which are made available in the laboratory by the instructor. You should concentrate on the similarities in form that permit identification of the components irrespective of cell type.

Note: When comparing sizes from one micrograph to another, remember to consider the respective magnifications.

Martens Dr Black Martens Martens Grizzly Dr Dr xwR7q0SxU Micrograph of a nucleus.

1. Heterochromatin
2. Euchromatin
3. Nucleolus
4. Nucleolar associated chromatin
5. Nuclear envelope

Salomon Speedcross Women's Pro Running Trail SS16 Shoes Blue OOfHr8R Micrograph of a portion of a nucleus:

What is the round structure (approximately 3 1/2 inches in diameter) seen in the center of this micrograph?

1. Nucleolar associated chromatin
2. Fibrillar component of nucleolus
3. Granular component of nucleolus
4. Euchromatin
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5. Heterochromatin
6. Nuclear pore
7. Nuclear envelope

Figure 3 Part of the nucleus and cytoplasm of a cell (at very high magnification): Pump Black Jessica Dress Women's Simpson Cirrus PIFF84B

Rustic Blundstone Chelsea 1490 Boot Black 1. Nuclear pore
2. Nuclear cisterna
3. Ribosomes
4. Heterochromatin

Figure 4 Blundstone Black Boot Chelsea 1490 Rustic A tangential section through the periphery of a nucleus (at a lower magnification than figure 3):

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1. Nuclear pores (Notice their shape in this en face section.)
2. Heterochromatin
3. Ribosomes

Figure 5 Portions of two cells are illustrated in this micrograph:

1. Two plasma membranes
2. Microtubule
1490 Chelsea Rustic Boot Blundstone Black 3. Nucleus
4. Golgi apparatus
a. Cisterna
b. vesicles
c. Vacuoles
5. Rough endoplasmic reticulum
6. Mitochondria

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1. Lipid droplet
2. Smooth endoplasmic reticulum
3. Mitochondria
4. Mitochondrial matrix granules
5. Cristae

Figure 7 Boot Rustic Black 1490 Blundstone Chelsea A cell with an abundance of smooth endoplasmic reticulum:

Compare the appearance and amount of the smooth endoplasmic reticulum in this micrograph with that shown in figure 6.

What are several functions of smooth endoplasmic reticulum?

Figure 8 1490 Blundstone Black Chelsea Rustic Boot An area of cytoplasm is illustrated:

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1. Mitochondria (Notice the structure of cristae as compared to Figure 6 )
2. Ribosomes
3. Lysosomes

Running Shoes Sugar Women Skull size And Lotus 7 5 Weighted Yellow nBYXqwEw An autophagic vacuole (at high magnification):

This autophagic vacuole contains a mitochondrion. The vacuole contents will most likely be extruded by exocytosis or persist to form a residual body.

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1. Mitochondria
2. Peroxisome
Chelsea Rustic Blundstone Boot Black 1490 3. Nucleoid
4. Rough endoplasmic reticulum
5. Glycogen

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1. Glycogen (Densely stained here versus unstained in Figure 10 )
2. Mitochondria
3. Peroxisome

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1. Two multivesicular bodies
2. Filaments
3. Mitochondrion
4. Lysosome

Non Grey M Pool Slip Slipper Shower Household Spa Beach Men Bathroom Comfortable Sandal Triple Plus ZSUxF Epithelial specializations (at very high magnification):

1. Unit membrane
Black Boot Blundstone Chelsea Rustic 1490 2. Glycocalyx
3. Microvillus (Note the black central actin filaments)
4. Coated vesicle
5. Multivesicular body
6. Desmosome
7. Filaments
Chelsea Black Blundstone Boot 1490 Rustic 8. Microtubule
9. Intercellular space
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Figure 14 1490 Black Chelsea Blundstone Boot Rustic Junctional specializations between two epithelial cells:

1. Zonula occludens (tight junction)
2. Zonula adherens (intermediate junction)
3. Macula adherens (desmosome)
Insert: Zonula occludens

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This micrograph illustrates a desmosome with its cytoplasmic plaques (B).

Figure 16 A gap junction:

The two unit membranes making up this gap junction are labelled C.

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This micrograph represents a cross section of a centriole. The microtubules are shown in their characteristic arrangement.

Figure 19 Blundstone Boot 1490 Chelsea Rustic Black Two centrioles are illustrated in this figure:

Longitudinal section of two centrioles.

Figure 20 Sneaker Women's Converse All Fashion Fringe Star High Grey Top Leather 8HOwHqP7x Cilia:

A represents a microtubule. Remember that 9 doublets form the periphery of the axoneme. Also know that a centriole has the 9 + 0 arrangement where a cilium is organized as 9 + 2.

Figure 21 Epithelial cells often display extensive basal plasma membrane infoldings as observed in this electron micrograph:

1. Compartmentalized mitochondrion
2. Extracellular space
3. Basal lamina
4. Heterochromatin

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1. Microtubule
2. Chromosomes

Figure 23 A cell in mitosis (Telophase):

1. Daughter nucleus
2. Midbody

Figure 24 Black 1490 Boot Chelsea Rustic Blundstone Striated muscle.

Note myosin filaments (unlabelled arrows), the A band (A), Z line (Z), the I band (I), and triads consisting of one T tubule (T) and two terminal cisternae (C), Myofibril (M), and Sarcolemma (S).

'Silver US 2017 QS Bullet AIR Max 97 Nike OG Release' 8SwXRaxq Intestinal epithelial absorptive cell.

Note the microvilli (1490 Boot Rustic Blundstone Chelsea Black MV) connected by junctional complexes (JC). Observe the lateral cell compartment (LC) between the cells.

Figure 26 Small intestine.

Note the microvilli (M) on the luminal surface of the two adjacent absorptive cells (A & B). The mircrovilli are coated with a glycocalyx (G).

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Note prominent secondary lysosomes (white arrows) and mitochondria (M). A few cisterna of rough endoplasmic reticulum (R) and microtubules (T) are also present.

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Observe the motochondria (M), granules (arrow), cristae (C), smooth (S) and rough (R) endoplasmic reticulum. Also present are numerous vesicles (V), a peroxisome (P), a bile canaliculus (B), and a cell junction (J).

Figure 29 Scanning EM of liver.

Examine bile canaliculi (b), sinusoids (S), the sinusoidal lining (SL), and the space of Disse (D)

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Examine numerous pinocytic vesicles (P), ribosomes (R), and mitochondria (M).

**EMs were selected and copied from "A Study Atlas of Electron Micrographs" by Judy M. Strum, Ph.D. with permission of the author. Dr. Strum is a Professor in the Department of Anatomy, University of Maryland School of Medicine.