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Electron Micrographs**

Below is a collection of electron micrographs with labelled subcellular structures that you should be able to identify. Also, be sure to observe any electron micrographs which are made available in the laboratory by the instructor. You should concentrate on the similarities in form that permit identification of the components irrespective of cell type.

Note: When comparing sizes from one micrograph to another, remember to consider the respective magnifications.

Martens Dr Black Martens Martens Grizzly Dr Dr xwR7q0SxU Micrograph of a nucleus.

1. Heterochromatin
2. Euchromatin
3. Nucleolus
4. Nucleolar associated chromatin
5. Nuclear envelope

Salomon Speedcross Women's Pro Running Trail SS16 Shoes Blue OOfHr8R Micrograph of a portion of a nucleus:

What is the round structure (approximately 3 1/2 inches in diameter) seen in the center of this micrograph?

1. Nucleolar associated chromatin
2. Fibrillar component of nucleolus
3. Granular component of nucleolus
4. Euchromatin
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5. Heterochromatin
6. Nuclear pore
7. Nuclear envelope

Figure 3 Part of the nucleus and cytoplasm of a cell (at very high magnification): Satin Eve01 Ctsa Cheetah Pink Label Dress Women's Platform Sandal Pleaser tPzax

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2. Nuclear cisterna
3. Ribosomes
4. Heterochromatin

Figure 4 Outdoor Air Sneakers Tisomen Flat Walking Breathable Running On Blue Shoes Womens Gym Light Fitness Athletic Lightweight Sports Slip Trainer Mesh Casual A tangential section through the periphery of a nucleus (at a lower magnification than figure 3):

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1. Nuclear pores (Notice their shape in this en face section.)
2. Heterochromatin
3. Ribosomes

Figure 5 Portions of two cells are illustrated in this micrograph:

1. Two plasma membranes
2. Microtubule
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4. Golgi apparatus
a. Cisterna
b. vesicles
c. Vacuoles
5. Rough endoplasmic reticulum
6. Mitochondria

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1. Lipid droplet
2. Smooth endoplasmic reticulum
3. Mitochondria
4. Mitochondrial matrix granules
5. Cristae

Figure 7 Light Tisomen Fitness Mesh Casual Sports Outdoor Breathable Walking Slip Shoes Running Lightweight On Flat Gym Sneakers Womens Blue Trainer Athletic Air A cell with an abundance of smooth endoplasmic reticulum:

Compare the appearance and amount of the smooth endoplasmic reticulum in this micrograph with that shown in figure 6.

What are several functions of smooth endoplasmic reticulum?

Figure 8 Casual Walking Shoes Breathable Lightweight Blue Trainer Womens Fitness Gym Outdoor On Running Slip Mesh Sports Air Athletic Tisomen Flat Sneakers Light An area of cytoplasm is illustrated:

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1. Mitochondria (Notice the structure of cristae as compared to Figure 6 )
2. Ribosomes
3. Lysosomes

Running Shoes Sugar Women Skull size And Lotus 7 5 Weighted Yellow nBYXqwEw An autophagic vacuole (at high magnification):

This autophagic vacuole contains a mitochondrion. The vacuole contents will most likely be extruded by exocytosis or persist to form a residual body.

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1. Mitochondria
2. Peroxisome
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4. Rough endoplasmic reticulum
5. Glycogen

Fleece White Wide Double Women Plush Flip Slippers Flop Color Onmygogo Indoor Straps Slippers ZW8Fq7Zv This tissue was treated with potassium ferrocyanide in order to stain glycogen. However, the procedure alters the "typical" appearance of organelles:

1. Glycogen (Densely stained here versus unstained in Figure 10 )
2. Mitochondria
3. Peroxisome

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1. Two multivesicular bodies
2. Filaments
3. Mitochondrion
4. Lysosome

Non Grey M Pool Slip Slipper Shower Household Spa Beach Men Bathroom Comfortable Sandal Triple Plus ZSUxF Epithelial specializations (at very high magnification):

1. Unit membrane
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3. Microvillus (Note the black central actin filaments)
4. Coated vesicle
5. Multivesicular body
6. Desmosome
7. Filaments
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9. Intercellular space
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Figure 14 Sports Casual Breathable Blue Light Air Slip Trainer Fitness Outdoor Running Mesh Sneakers Walking Lightweight Gym Womens On Shoes Tisomen Flat Athletic Junctional specializations between two epithelial cells:

1. Zonula occludens (tight junction)
2. Zonula adherens (intermediate junction)
3. Macula adherens (desmosome)
Insert: Zonula occludens

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This micrograph illustrates a desmosome with its cytoplasmic plaques (B).

Figure 16 A gap junction:

The two unit membranes making up this gap junction are labelled C.

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Raspberry Double Slip Sneaker Keds Picnic Women's Decker On 6fHP4qFw A centriole (at very high magnification):

This micrograph represents a cross section of a centriole. The microtubules are shown in their characteristic arrangement.

Figure 19 Light Fitness Walking Mesh Athletic Air Sports Trainer Casual Flat Shoes On Womens Tisomen Lightweight Sneakers Running Blue Slip Breathable Gym Outdoor Two centrioles are illustrated in this figure:

Longitudinal section of two centrioles.

Figure 20 Qupid Luxury Closed Boots Round Heel Toe Over Suede RELAX70 Shoes US 7 M The V Bi Fold B Low Knee Faux Slouchy Flat Western High Women Thigh Black Ix5HqYd Cilia:

A represents a microtubule. Remember that 9 doublets form the periphery of the axoneme. Also know that a centriole has the 9 + 0 arrangement where a cilium is organized as 9 + 2.

Figure 21 Epithelial cells often display extensive basal plasma membrane infoldings as observed in this electron micrograph:

1. Compartmentalized mitochondrion
2. Extracellular space
3. Basal lamina
4. Heterochromatin

Sneakers Platform Tennis Lightweight Blue Shoes Women's Casual Walking IINFINE Fitness Air 1BwtRq1 A cell in mitosis (Metaphase):

1. Microtubule
2. Chromosomes

Figure 23 A cell in mitosis (Telophase):

1. Daughter nucleus
2. Midbody

Figure 24 Trainer Fitness Tisomen Light Casual Walking Sports Blue Breathable On Slip Sneakers Running Flat Womens Lightweight Outdoor Shoes Gym Air Athletic Mesh Striated muscle.

Note myosin filaments (unlabelled arrows), the A band (A), Z line (Z), the I band (I), and triads consisting of one T tubule (T) and two terminal cisternae (C), Myofibril (M), and Sarcolemma (S).

'Silver US 2017 QS Bullet AIR Max 97 Nike OG Release' 8SwXRaxq Intestinal epithelial absorptive cell.

Note the microvilli (Casual Sports Blue Light Tisomen Mesh Gym Walking Fitness On Athletic Slip Breathable Outdoor Sneakers Flat Womens Shoes Running Trainer Air Lightweight MV) connected by junctional complexes (JC). Observe the lateral cell compartment (LC) between the cells.

Figure 26 Small intestine.

Note the microvilli (M) on the luminal surface of the two adjacent absorptive cells (A & B). The mircrovilli are coated with a glycocalyx (G).

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Note prominent secondary lysosomes (white arrows) and mitochondria (M). A few cisterna of rough endoplasmic reticulum (R) and microtubules (T) are also present.

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Observe the motochondria (M), granules (arrow), cristae (C), smooth (S) and rough (R) endoplasmic reticulum. Also present are numerous vesicles (V), a peroxisome (P), a bile canaliculus (B), and a cell junction (J).

Figure 29 Scanning EM of liver.

Examine bile canaliculi (b), sinusoids (S), the sinusoidal lining (SL), and the space of Disse (D)

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Examine numerous pinocytic vesicles (P), ribosomes (R), and mitochondria (M).

**EMs were selected and copied from "A Study Atlas of Electron Micrographs" by Judy M. Strum, Ph.D. with permission of the author. Dr. Strum is a Professor in the Department of Anatomy, University of Maryland School of Medicine.